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Posted on April 10, 2024
When should I use fluorescence detection methods for western blot?
Antibodies and Proteomics BioconjugationPrimary AntibodiesSecondary AntibodiesWestern Blot
When should I use fluorescence detection methods for western blot?
Antibodies and Proteomics BioconjugationPrimary AntibodiesSecondary AntibodiesWestern Blot
Posted on April 10, 2024
What are the disadvantages of fluorescence detection method for western blot?
Antibodies and Proteomics BioconjugationFluorescencePrimary AntibodiesSecondary AntibodiesWestern Blot
What are the disadvantages of fluorescence detection method for western blot?
Antibodies and Proteomics BioconjugationFluorescencePrimary AntibodiesSecondary AntibodiesWestern Blot
Posted on April 10, 2024
What are the advantages of fluorescence detection methods for western blot?
Antibodies and Proteomics BioconjugationFluorescencePrimary AntibodiesSecondary AntibodiesWestern Blot
What are the advantages of fluorescence detection methods for western blot?
Antibodies and Proteomics BioconjugationFluorescencePrimary AntibodiesSecondary AntibodiesWestern Blot
Posted on April 10, 2024
When should I use a chemiluminescent detection method for western blot?
Antibodies and Proteomics BioconjugationPrimary AntibodiesSecondary AntibodiesWestern Blot
When should I use a chemiluminescent detection method for western blot?
Antibodies and Proteomics BioconjugationPrimary AntibodiesSecondary AntibodiesWestern Blot
Posted on April 10, 2024
What are the disadvantages of Chemiluminescent detection method for western blot?
Antibodies and Proteomics BioconjugationPrimary AntibodiesSecondary AntibodiesWestern Blot
What are the disadvantages of Chemiluminescent detection method for western blot?
Antibodies and Proteomics BioconjugationPrimary AntibodiesSecondary AntibodiesWestern Blot
Posted on April 10, 2024
What are the advantages of Chemiluminescent detection method for western blot?
Antibodies and Proteomics BioconjugationPrimary AntibodiesSecondary AntibodiesWestern Blot
What are the advantages of Chemiluminescent detection method for western blot?
Antibodies and Proteomics BioconjugationPrimary AntibodiesSecondary AntibodiesWestern Blot
Posted on April 10, 2024
When should I use colorimetric detection for western blot?
Antibodies and Proteomics BioconjugationPrimary AntibodiesSecondary AntibodiesWestern Blot
When should I use colorimetric detection for western blot?
Antibodies and Proteomics BioconjugationPrimary AntibodiesSecondary AntibodiesWestern Blot
Posted on April 10, 2024
What are the disadvantages of colorimetric detection for western blot?
Antibodies and Proteomics BioconjugationPrimary AntibodiesSecondary AntibodiesWestern Blot
What are the disadvantages of colorimetric detection for western blot?
Antibodies and Proteomics BioconjugationPrimary AntibodiesSecondary AntibodiesWestern Blot
Posted on April 10, 2024
What are the advantages of colorimetric detection for western blot?
Antibodies and Proteomics BioconjugationPrimary AntibodiesSecondary AntibodiesWestern Blot
What are the advantages of colorimetric detection for western blot?
Antibodies and Proteomics BioconjugationPrimary AntibodiesSecondary AntibodiesWestern Blot
Posted on April 10, 2024
What are the most commonly used enzymes in colorimetric detection for western blot?
Antibodies and Proteomics BioconjugationPrimary AntibodiesSecondary AntibodiesWestern Blot
What are the most commonly used enzymes in colorimetric detection for western blot?
Antibodies and Proteomics BioconjugationPrimary AntibodiesSecondary AntibodiesWestern Blot
Posted on April 10, 2024
How do I choose the right western blot detection method?
Antibodies and Proteomics BioconjugationPrimary AntibodiesSecondary AntibodiesWestern Blot
How do I choose the right western blot detection method?
Antibodies and Proteomics BioconjugationPrimary AntibodiesSecondary AntibodiesWestern Blot
Posted on April 10, 2024
What are the disadvantages of the radioactive detection method for western blot?
Antibodies and Proteomics BioconjugationPrimary AntibodiesSecondary AntibodiesWestern Blot
What are the disadvantages of the radioactive detection method for western blot?
Antibodies and Proteomics BioconjugationPrimary AntibodiesSecondary AntibodiesWestern Blot
Posted on April 10, 2024
What should I do if I have a high background (uniform distribution) in western blot?
Antibodies and Proteomics BioconjugationPrimary AntibodiesSecondary AntibodiesWestern Blotimmunofluorescence
What should I do if I have a high background (uniform distribution) in western blot?
Antibodies and Proteomics BioconjugationPrimary AntibodiesSecondary AntibodiesWestern Blotimmunofluorescence
Posted on April 10, 2024
What should I do to get optimal transfer results?
Antibodies and Proteomics BioconjugationPrimary AntibodiesSecondary Antibodiesimmunofluorescence
What should I do to get optimal transfer results?
Antibodies and Proteomics BioconjugationPrimary AntibodiesSecondary Antibodiesimmunofluorescence
Posted on April 10, 2024
What roles do SDS and alcohol play in the western blot transfer step?
Antibodies and Proteomics BioconjugationPrimary AntibodiesSecondary AntibodiesWestern Blotimmunofluorescence
What roles do SDS and alcohol play in the western blot transfer step?
Antibodies and Proteomics BioconjugationPrimary AntibodiesSecondary AntibodiesWestern Blotimmunofluorescence
Posted on April 10, 2024
What are the different types of transfer methods in western blot?
Antibodies and Proteomics BioconjugationPrimary AntibodiesSecondary AntibodiesWestern Blotimmunofluorescence
What are the different types of transfer methods in western blot?
Antibodies and Proteomics BioconjugationPrimary AntibodiesSecondary AntibodiesWestern Blotimmunofluorescence
Posted on April 10, 2024
How do I validate the transfer?
Antibodies and Proteomics BioconjugationPrimary AntibodiesSecondary Antibodiesimmunofluorescence
How do I validate the transfer?
Antibodies and Proteomics BioconjugationPrimary AntibodiesSecondary Antibodiesimmunofluorescence
Posted on April 10, 2024
Why is the data produced with a western blot typically considered to be semi-quantitative?
Antibodies and Proteomics BioconjugationPrimary AntibodiesSecondary AntibodiesWestern Blotimmunofluorescence
Why is the data produced with a western blot typically considered to be semi-quantitative?
Antibodies and Proteomics BioconjugationPrimary AntibodiesSecondary AntibodiesWestern Blotimmunofluorescence
Posted on April 10, 2024
What are the applications of southern blotting?
Antibodies and Proteomics BioconjugationDNA and RNA QuantitationSecondary AntibodiesWestern Blotimmunofluorescence
What are the applications of southern blotting?
Antibodies and Proteomics BioconjugationDNA and RNA QuantitationSecondary AntibodiesWestern Blotimmunofluorescence
Posted on April 10, 2024
What are the steps involved in southern blotting?
DNA and RNA QuantitationEnzymesGel ElectrophoresisPrimary AntibodiesSecondary AntibodiesWestern Blot
What are the steps involved in southern blotting?
DNA and RNA QuantitationEnzymesGel ElectrophoresisPrimary AntibodiesSecondary AntibodiesWestern Blot
Posted on April 10, 2024
What are the principles of southern blotting?
Antibodies and Proteomics BioconjugationPrimary AntibodiesSecondary AntibodiesWestern Blotimmunofluorescence
What are the principles of southern blotting?
Antibodies and Proteomics BioconjugationPrimary AntibodiesSecondary AntibodiesWestern Blotimmunofluorescence
Posted on April 10, 2024
What are the principles of western blotting?
Antibodies and Proteomics BioconjugationPrimary AntibodiesSecondary AntibodiesWestern Blotimmunofluorescence
What are the principles of western blotting?
Antibodies and Proteomics BioconjugationPrimary AntibodiesSecondary AntibodiesWestern Blotimmunofluorescence
Posted on April 10, 2024
What are the applications of western blotting?
Antibodies and Proteomics BioconjugationSecondary AntibodiesWestern Blotimmunofluorescence
What are the applications of western blotting?
Antibodies and Proteomics BioconjugationSecondary AntibodiesWestern Blotimmunofluorescence
Posted on April 10, 2024
What do I need for my western blot membrane blotting?
Antibodies and Proteomics BioconjugationSecondary AntibodiesWestern Blotimmunofluorescence
What do I need for my western blot membrane blotting?
Antibodies and Proteomics BioconjugationSecondary AntibodiesWestern Blotimmunofluorescence
Posted on April 10, 2024
What are the disadvantages of nylon membranes?
Antibodies and Proteomics BioconjugationSecondary AntibodiesWestern Blotimmunofluorescence
What are the disadvantages of nylon membranes?
Antibodies and Proteomics BioconjugationSecondary AntibodiesWestern Blotimmunofluorescence
Posted on April 10, 2024
What are the advantages of nylon membranes?
Antibodies and Proteomics BioconjugationSecondary AntibodiesWestern Blotimmunofluorescence
What are the advantages of nylon membranes?
Antibodies and Proteomics BioconjugationSecondary AntibodiesWestern Blotimmunofluorescence
Posted on April 10, 2024
What are the differences between nitrocellulose membrane and nylon membrane?
Antibodies and ProteomicsPhysiological ProbesSecondary AntibodiesWestern Blotimmunofluorescence
What are the differences between nitrocellulose membrane and nylon membrane?
Antibodies and ProteomicsPhysiological ProbesSecondary AntibodiesWestern Blotimmunofluorescence
Posted on April 10, 2024
How do I optimize my western blot experiments?
Antibodies and Proteomics BioconjugationSecondary AntibodiesWestern Blotimmunofluorescence
How do I optimize my western blot experiments?
Antibodies and Proteomics BioconjugationSecondary AntibodiesWestern Blotimmunofluorescence
Posted on April 10, 2024
What are the common causes of an unexpected size of western blot band?
Antibodies and Proteomics BioconjugationSecondary AntibodiesWestern Blotimmunofluorescence
What are the common causes of an unexpected size of western blot band?
Antibodies and Proteomics BioconjugationSecondary AntibodiesWestern Blotimmunofluorescence
Posted on April 10, 2024
What should I take into consideration before I strip and reprobe my western blotting experiment?
Antibodies and Proteomics BioconjugationSecondary AntibodiesWestern Blotimmunofluorescence
What should I take into consideration before I strip and reprobe my western blotting experiment?
Antibodies and Proteomics BioconjugationSecondary AntibodiesWestern Blotimmunofluorescence
Posted on March 11, 2024
Why do we need controls for immunofluorescence?
Antibodies and Proteomics BioconjugationAntibody and Protein LabelingPrimary AntibodiesSecondary Antibodiesimmunofluorescence
Why do we need controls for immunofluorescence?
Antibodies and Proteomics BioconjugationAntibody and Protein LabelingPrimary AntibodiesSecondary Antibodiesimmunofluorescence
Posted on March 11, 2024
What do the different types of controls for immunofluorescence do?
Antibodies and Proteomics BioconjugationAntibody and Protein LabelingControlsPrimary AntibodiesSecondary Antibodiesimmunofluorescence
What do the different types of controls for immunofluorescence do?
Antibodies and Proteomics BioconjugationAntibody and Protein LabelingControlsPrimary AntibodiesSecondary Antibodiesimmunofluorescence
Posted on October 25, 2022
What are the detection methods for western blot?
Enzyme-Linked Immunosorbent Assay (ELISA)Antibody and Protein LabelingImmunohistochemistry (IHC)Secondary AntibodiesWestern Blot
What are the detection methods for western blot?
Enzyme-Linked Immunosorbent Assay (ELISA)Antibody and Protein LabelingImmunohistochemistry (IHC)Secondary AntibodiesWestern Blot
Posted on October 25, 2022
What are the steps involved in western blot?
Bioconjugation Enzyme-Linked Immunosorbent Assay (ELISA)Antibody and Protein LabelingHorseradish Peroxidase (HRP) and Poly-HRPSecondary AntibodiesWestern Blot
What are the steps involved in western blot?
Bioconjugation Enzyme-Linked Immunosorbent Assay (ELISA)Antibody and Protein LabelingHorseradish Peroxidase (HRP) and Poly-HRPSecondary AntibodiesWestern Blot
Posted on August 5, 2022
What are the differences between IgG, IgM, IgE and IgD?
Antibody and Protein LabelingSecondary AntibodiesImmunohistochemistry (IHC)Bioconjugation
What are the differences between IgG, IgM, IgE and IgD?
Antibody and Protein LabelingSecondary AntibodiesImmunohistochemistry (IHC)Bioconjugation
Posted on September 29, 2021
What is secondary antibody cross reactivity?
Secondary AntibodiesAntibodies and Proteomics
What is secondary antibody cross reactivity?
Secondary AntibodiesAntibodies and Proteomics
Posted on September 28, 2021
What is the concentration of secondary antibodies for immunofluorescence?
Secondary AntibodiesAntibodies and ProteomicsImmunohistochemistry (IHC)Power Styramide™ Signal Amplification (PSA™)
What is the concentration of secondary antibodies for immunofluorescence?
Secondary AntibodiesAntibodies and ProteomicsImmunohistochemistry (IHC)Power Styramide™ Signal Amplification (PSA™)
Posted on September 28, 2021
What does cross adsorbed secondary antibody mean?
Secondary AntibodiesAntibodies and ProteomicsPrimary AntibodiesIndocyanine Green
What does cross adsorbed secondary antibody mean?
Secondary AntibodiesAntibodies and ProteomicsPrimary AntibodiesIndocyanine Green
Posted on September 28, 2021
How long do secondary antibodies last?
Secondary AntibodiesAntibodies and Proteomics
How long do secondary antibodies last?
Secondary AntibodiesAntibodies and Proteomics
Posted on September 28, 2021
What is the role of secondary antibodies?
Secondary AntibodiesPrimary AntibodiesImmunohistochemistry (IHC)Enzyme-Linked Immunosorbent Assay (ELISA)
What is the role of secondary antibodies?
Secondary AntibodiesPrimary AntibodiesImmunohistochemistry (IHC)Enzyme-Linked Immunosorbent Assay (ELISA)
Posted on September 27, 2021
How do I know if an antibody is primary or secondary?
EnzymesSecondary AntibodiesAntibodies and ProteomicsHorseradish Peroxidase (HRP) and Poly-HRP
How do I know if an antibody is primary or secondary?
EnzymesSecondary AntibodiesAntibodies and ProteomicsHorseradish Peroxidase (HRP) and Poly-HRP
Posted on September 27, 2021
What does a conjugated antibody mean?
Horseradish Peroxidase (HRP) and Poly-HRPPE and APCSecondary AntibodiesAntibodies and Proteomics
What does a conjugated antibody mean?
Horseradish Peroxidase (HRP) and Poly-HRPPE and APCSecondary AntibodiesAntibodies and Proteomics
Posted on September 27, 2021
What is the advantage of using the pre-adsorbed secondary antibody?
Secondary AntibodiesAntibodies and ProteomicsImmunohistochemistry (IHC)
What is the advantage of using the pre-adsorbed secondary antibody?
Secondary AntibodiesAntibodies and ProteomicsImmunohistochemistry (IHC)
Posted on September 24, 2021
What IgG class or subclass should I use for my primary antibody?
Protein to Protein ConjugationSecondary AntibodiesPrimary AntibodiesAntibody and Protein Labeling
What IgG class or subclass should I use for my primary antibody?
Protein to Protein ConjugationSecondary AntibodiesPrimary AntibodiesAntibody and Protein Labeling
Posted on September 24, 2021
What is pre-adsorption?
Secondary AntibodiesPE and APCBioconjugationProtein to Protein Conjugation
What is pre-adsorption?
Secondary AntibodiesPE and APCBioconjugationProtein to Protein Conjugation
Posted on September 23, 2021
What is the difference between cross adsorbed/normal/purified antibodies?
Antibodies and ProteomicsSecondary Antibodies
What is the difference between cross adsorbed/normal/purified antibodies?
Antibodies and ProteomicsSecondary Antibodies
Posted on September 23, 2021
What are the major benefits of using a secondary antibody
Antibodies and ProteomicsPrimary AntibodiesSecondary Antibodies
What are the major benefits of using a secondary antibody
Antibodies and ProteomicsPrimary AntibodiesSecondary Antibodies
Posted on September 22, 2021
What label should I choose for a secondary antibody?
Secondary AntibodiesAntibodies and ProteomicsImmunohistochemistry (IHC)
What label should I choose for a secondary antibody?
Secondary AntibodiesAntibodies and ProteomicsImmunohistochemistry (IHC)
Posted on September 22, 2021
What is the most important criterion for choosing a secondary antibody?
Secondary AntibodiesAntibodies and Proteomics
What is the most important criterion for choosing a secondary antibody?
Secondary AntibodiesAntibodies and Proteomics
Posted on September 22, 2021
What are the common applications for secondary antibody conjugates?
Secondary AntibodiesBioconjugationImmunohistochemistry (IHC)Enzyme-Linked Immunosorbent Assay (ELISA)Antibody and Protein Labeling
What are the common applications for secondary antibody conjugates?
Secondary AntibodiesBioconjugationImmunohistochemistry (IHC)Enzyme-Linked Immunosorbent Assay (ELISA)Antibody and Protein Labeling
Posted on September 22, 2021
How do I choose secondary antibodies?
Secondary AntibodiesAntibodies and ProteomicsHorseradish Peroxidase (HRP) and Poly-HRPEnzyme-Linked Immunosorbent Assay (ELISA)
How do I choose secondary antibodies?
Secondary AntibodiesAntibodies and ProteomicsHorseradish Peroxidase (HRP) and Poly-HRPEnzyme-Linked Immunosorbent Assay (ELISA)
Posted on September 22, 2021
What is HRP conjugated secondary antibody?
EnzymesSecondary AntibodiesHorseradish Peroxidase (HRP) and Poly-HRPPower Styramide™ Signal Amplification (PSA™)
What is HRP conjugated secondary antibody?
EnzymesSecondary AntibodiesHorseradish Peroxidase (HRP) and Poly-HRPPower Styramide™ Signal Amplification (PSA™)
Posted on September 22, 2021
Why is an HRP conjugated secondary antibody used in western blotting?
Horseradish Peroxidase (HRP) and Poly-HRPEnzymesSecondary AntibodiesAntibodies and Proteomics
Why is an HRP conjugated secondary antibody used in western blotting?
Horseradish Peroxidase (HRP) and Poly-HRPEnzymesSecondary AntibodiesAntibodies and Proteomics
Posted on September 22, 2021
What are the major differences between primary and secondary antibodies?
Secondary AntibodiesPrimary AntibodiesAntibody and Protein Labeling
What are the major differences between primary and secondary antibodies?
Secondary AntibodiesPrimary AntibodiesAntibody and Protein Labeling
Posted on September 21, 2021
Is IgG a secondary antibody?
Secondary AntibodiesAntibodies and ProteomicsPower Styramide™ Signal Amplification (PSA™)
Is IgG a secondary antibody?
Secondary AntibodiesAntibodies and ProteomicsPower Styramide™ Signal Amplification (PSA™)
Posted on September 21, 2021
What do anti-human antibodies bind to?
Secondary AntibodiesAntibodies and Proteomics
What do anti-human antibodies bind to?
Secondary AntibodiesAntibodies and Proteomics
Posted on September 21, 2021
Are there different IgG antibodies?
Antibodies and ProteomicsImmunohistochemistry (IHC)Secondary Antibodies
Are there different IgG antibodies?
Antibodies and ProteomicsImmunohistochemistry (IHC)Secondary Antibodies
Posted on September 21, 2021
What do anti-human antibodies do?
Secondary AntibodiesAntibodies and ProteomicsHorseradish Peroxidase (HRP) and Poly-HRP
What do anti-human antibodies do?
Secondary AntibodiesAntibodies and ProteomicsHorseradish Peroxidase (HRP) and Poly-HRP
Posted on September 21, 2021
What is anti human IgG?
Secondary AntibodiesAntibodies and ProteomicsHorseradish Peroxidase (HRP) and Poly-HRP
What is anti human IgG?
Secondary AntibodiesAntibodies and ProteomicsHorseradish Peroxidase (HRP) and Poly-HRP
Posted on September 20, 2021
What are IgG antibodies used for?
Antibodies and ProteomicsAntibody and Protein LabelingSecondary Antibodies
What are IgG antibodies used for?
Antibodies and ProteomicsAntibody and Protein LabelingSecondary Antibodies
Posted on June 1, 2020
What is the difference between direct and indirect ELISA?
Antibodies and Proteomics Enzyme-Linked Immunosorbent Assay (ELISA)Antibody and Protein LabelingDirect ELISAPrimary AntibodiesSecondary Antibodies
What is the difference between direct and indirect ELISA?
Antibodies and Proteomics Enzyme-Linked Immunosorbent Assay (ELISA)Antibody and Protein LabelingDirect ELISAPrimary AntibodiesSecondary Antibodies
Posted on April 24, 2020
Why does a secondary antibody have to originate from a host species different than that of the primary antibody?
Antibodies and Proteomics BioconjugationAntibody and Protein LabelingImmunohistochemistry (IHC)Primary AntibodiesSecondary Antibodies
Why does a secondary antibody have to originate from a host species different than that of the primary antibody?
Antibodies and Proteomics BioconjugationAntibody and Protein LabelingImmunohistochemistry (IHC)Primary AntibodiesSecondary Antibodies
Posted on April 24, 2020
What are the criteria for choosing primary and secondary antibodies in western blotting?
Antibodies and ProteomicsAntibody and Protein LabelingPrimary AntibodiesSecondary AntibodiesWestern Blot
What are the criteria for choosing primary and secondary antibodies in western blotting?
Antibodies and ProteomicsAntibody and Protein LabelingPrimary AntibodiesSecondary AntibodiesWestern Blot
Posted on April 24, 2020
Can I use an HRP-conjugated primary antibody for western blot (with no secondary)?
Antibodies and Proteomics Bioconjugation Chemical ReagentsAntibody and Protein LabelingHRPHRP-labeled primary antibodyHorseradish Peroxidase (HRP) and Poly-HRPPrimary AntibodiesSecondary AntibodiesWestern Blotantibody conjugation
Can I use an HRP-conjugated primary antibody for western blot (with no secondary)?
Antibodies and Proteomics Bioconjugation Chemical ReagentsAntibody and Protein LabelingHRPHRP-labeled primary antibodyHorseradish Peroxidase (HRP) and Poly-HRPPrimary AntibodiesSecondary AntibodiesWestern Blotantibody conjugation
Posted on April 24, 2020
What is the difference between indirect and direct imaging?
Antibodies and ProteomicsAntibody and Protein LabelingIHCImmunohistochemistry (IHC)Primary AntibodiesSecondary Antibodiesimmunofluorescence
What is the difference between indirect and direct imaging?
Antibodies and ProteomicsAntibody and Protein LabelingIHCImmunohistochemistry (IHC)Primary AntibodiesSecondary Antibodiesimmunofluorescence